African city leaders gather in Joburg

first_img3 December 2015Local government was increasingly being identified as the strategic enabler of national economic and development objectives, said South African Local Government Association chairperson Thabo Manyoni, speaking on the first day of the seventh Africities Summit.Africities 7 Summit, as it is official known, is being held in Johannesburg and runs until 3 December. It brings together hundreds of city officials from across the continent, where they are unpacking and exploring the future of urbanisation in Africa. It is an important topic given that more than half of Africa’s population is expected to live in cities by the year 2050, according to Associated Press.Manyoni said the most obvious impact of the current global economic system was rising inequality and its socioeconomic impact. “It is said that Africa and Asia will account for 90% of urban growth over the next 35 years. They have very young populations, which represents a massive potential expansion of the labour force and middle class. This is a great advantage, if properly managed.”Only 28% of the labour force in Africa occupied stable wage-earning jobs, he said, compared to 63% in vulnerable employment, with over 60% of urban dwellers in sub-Saharan Africa currently living in informal settlements.“This means that potential tax bases of urban governments are relatively small, creating a serious financial imbalance to address the vast service delivery and economic infrastructure needs. This must inform a differentiated approach to tackling the development agenda in our context.”Leaders should not forget to invest in the development of small towns and rural villages, which were often the bedrock of agricultural wealth, heritage and cultural diversity, Manyoni added.“We have a responsibility to fashion a uniquely African response to social justice, equality in opportunity and sustainable infrastructure development and resource use.”The summit ties in with the National Development Plan, or Vision 2030, which places emphasis on overcoming the challenges of the present to build a better South Africa and Africa.It was opened by Minister Jeff Radebe, the minister in The Presidency responsible for planning. “What has worked yesterday, might not work today,” he said, placing the focus firmly on looking towards the future.Development had to match population growth or the cities would face more crumbling infrastructure and social unrest, Radebe said.Also part of the discussions over the five days will be climate change as the CoP21 takes place in Paris, as well as urban security following the recent attacks in Bamako, Garissa, Nairobi, Paris and other cities.The central theme of the Africities 7 Summit is “Shaping the future of Africa with the people: the contribution of African local authorities to Agenda 2063 of the African Union”.Agenda 2063 – Towards an Africa We Want encourages all people on the continent to play an active role to see the following vision come to fruition:We aspire that by 2063, Africa shall be a prosperous continent, with the means and resources to drive its own development;Where African people have a high standard of living, quality of life, sound health and well-being;A continent full of well-educated citizens through a skills revolution underpinned by science, technology and innovation for a knowledge society;Cities and other settlements are hubs of cultural and economic activities, with modernised infrastructure, and people have access to all the basic necessities of life including shelter, water, sanitation, energy, public transport and ICT; and,Economies are structurally transformed to create shared growth, decent jobs and economic opportunities for all.Taking its cue from Agenda 2063, organisers say the Africities Summit 7 theme is designed to connect a rigorous understanding of likely future trends with a strategic debate about what needs to be done at the local level, with immediate effect to address the emergency of service delivery, shelter, economic opportunities, safe and affordable mobility and more.The summit intends to be the mouthpiece of the 15 000 African local governments. More than 5 000 local government officials are participating, representing all the stakeholders of African local life as well as their partners of the other regions of the world. The Africities 7 Exhibition is hosting between 400 and 500 exhibitors.The Africities Summit pursues two major objectives:Defining appropriate shared strategies in order to improve the living conditions of the people at local level; and,Contributing to the integration, peace and unity of Africa starting from the grassroots.The meeting has been held in various African cities every three years since it was launched in 1998 in Abidjan, the largest city of Ivory reporterlast_img read more

No infighting in BJD, party not weak: Naveen

first_imgBJD president and Odisha Chief Minister Naveen Patnaik has said that there is no infighting in the party.“I do not feel any infighting in the party. Where is infighting?” Patnaik aksed while replying reporters on his return from his five-day visit to the national capital.When the Chief Minister was asked whether infighting in the regional outfit has been making the party weak, he said .“Who says the party is going weak? I don’t think its going weak at all? We are the largest party in the state. and have won four terms in a row.” When his attention was drawn to a news article written by BJD MP Bhartuhari Mahatab where he indicated infighting in BJD, .Patnaik said he has not gone through the article.On his visit to the residence of former prime minister A B Vajpayee during his stay in Delhi, Patnaik said “I heard Mr Vajpayee was not feeling well. So I paid a courtesy visit.” On claims made by a section of BJP leaders in Odisha that he wanted an alliance with the saffron party ahead of the 2019 general elections, the Chief Minister said “That is not correct.” Asked whether he has any plans to join the non—BJP parties to field an united opposition candidate in the ensuing presidential elections, he said “There are no plans yet. There were no discussions in Delhi as far as I am concerned.” Replying to a question on the Maoist attack in Chhattisgarh and Union Home Minister Rajnath Singh’s proposed meeting with the chief ministers of Naxal—hit states, Patnaik said “It (the killing of 25 security personnel in Sukma) is very tragic. We will wait for the invitation for the May 8 meeting from the Union Home Minister on this.”last_img read more

Dineshwar Sharma reaches out to J&K parties

first_imgThe Centre’s Special Representative Dineshwar Sharma, who is on a three-day visit to Srinagar to interact with various stakeholders in Jammu and Kashmir (J&K), is reaching out to political parties in the State. He has so far met delegations that were sent invitations for talks by the State government.Mr. Sharma met representatives of the Janata Dal(U), Peoples Democratic Party (PDP) and Lok Jan Shakti Party (LJP) on Tuesday and is expected to meet Congress and CPI(M) representatives on Wednesday.A women’s delegation, the first of its kind, apprised Mr. Sharma of problems faced by women, while another delegation underlined the “urgency to talk to Pakistan and separatists”.The delegates said Mr. Sharma hinted at widening the dialogue to other stakeholders too, hinting at the possible inclusion of Hurriyat leaders. Thirty-six delegations met the Special Representative on Tuesday, including a group calling itself the ‘Rahul Gandhi Fans Association’.Channels blamedMr. Sharma has been tasked by the Centre to initiate and “carry forward a dialogue” with the elected representatives, various organizations and concerned individuals in J&K.Sanjay Saraf, spokesperson of Lok Janshakti Party (LJP) said some news channels were vilifying Kashmiris for TRPs and said this needs to be stopped.“We told the Special Representative that the youth of Kashmir has seen violence for the past 27 years and this needs to stop. The news channels are sending out a wrong message of Kashmir by portraying them in a negative light,” Mr. Saraf said.Abid Jan, president of the State Employees Working Women Association (SEWWA), said she met Mr. Sharma to focus concerns of women affected by the prolonged conflict.Bearing the brunt“We have 35,000 widows, especially in Kupwara. We have a huge chunk of women folk in Kunan Poshpora who are widows. We discussed about rehabilitation of women affected by the conflict,” she said.Calling for peace centres in every district in Kashmir, Ms Jan said they should be “headed by peace officers. Women also need a platform to put forth their view point.”Mushtaq Ahmad Lone, a Srinagar-based transporter who was part of one of the delegations, said prolonged strikes called by separatists had caused huge losses.“We don’t want that our people become militants and pick up guns. A one day strike impacts our business for the next four days, some people are maligning the entire State,” Mr. Lone said.last_img read more

Here we go: Just like old times, Federer vs. Nadal in France

first_imgView comments Catholic schools seek legislated pay hike, too Ethel Booba twits Mocha over 2 toilets in one cubicle at SEA Games venue The only time Federer won the French Open, in 2009, he didn’t need to face Nadal, who was knocked out in the fourth round by Robin Soderling. That’s one of only two losses for Nadal in 93 career matches at the place.There was very little drama in Nadal’s quarterfinal victory this time, a 6-1, 6-1, 6-3 stroll against No. 7 Kei Nishikori, who went through a pair of five-setters the past two rounds.“He didn’t let me play how I wanted to play,” Nishikori said, “and especially if I’m not fresh, it’s not easy to stay with him.”Federer vs. Wawrinka, meanwhile, lived up to the billing, a 3½-hour tussle between a couple of guys who won a Davis Cup title and Olympic gold medal in doubles together.Coming in, Federer held a 22-3 edge head-to-head, but Wawrinka knew this: All three of his victories had come on clay, including four years ago in the same round, on the same court.They were locked in a taut fourth set at 3-all when clouds grew thick and charcoal-colored. Thunder rumbled nearby. It was difficult to see, because there are no artificial lights. Play was suspended as a storm arrived. But it didn’t last long.When action resumed, Federer needed only 10 minutes to take control, getting his second break of the match by smacking a big cross-court forehand to a corner that Wawrinka couldn’t handle. It was 5-4, and Federer only needed to hold serve to end it.That turned out to be a tad complicated.He double-faulted for the only two times of the entire afternoon, once on his second match point. He was forced to deflect a break point for Wawrinka, doing so with a serve-and-volley winner. And then, finally, on his third match point, Federer closed it out with a volley, then hugged Wawrinka at the net.“A tough, tough one, for sure,” said Wawrinka, who was coming off the longest singles match of his career, a 5-hour, 9-minute victory over Stefanos Tsitsipas. Private companies step in to help SEA Games hosting Just like old times.Federer put away Stan Wawrinka after a 75-minute rain delay Tuesday, converting a break point for just the second time in 18 chances and quickly wrapping up a 7-6 (4), 4-6, 7-6 (5), 6-4 victory over his friend and Swiss countryman to reach the semifinals at Roland Garros.FEATURED STORIESSPORTSPrivate companies step in to help SEA Games hostingSPORTSPalace wants Cayetano’s PHISGOC Foundation probed over corruption chargesSPORTSSingapore latest to raise issue on SEA Games food, logisticsAnd what a showdown comes next: Federer vs. Nadal for a spot in the final.“Always,” Nadal said, “a big match.” LATEST STORIES DA eyes importing ‘galunggong’ anew MOST READ Two-day strike in Bicol fails to cripple transport Duterte wants probe of SEA Games messcenter_img Trending Articles PLAY LIST 00:50Trending Articles01:00Chief Justice Peralta on upcoming UAAP game: UP has no match against UST01:08Smash hit? Tesla suffers broken glass mishap at launch of new truck02:42PH underwater hockey team aims to make waves in SEA Games01:44Philippines marks anniversary of massacre with calls for justice01:19Fire erupts in Barangay Tatalon in Quezon City01:07Trump talks impeachment while meeting NCAA athletes02:49World-class track facilities installed at NCC for SEA Games02:11Trump awards medals to Jon Voight, Alison Krauss Sports Related Videospowered by AdSparcRead Next KEY BISCAYNE, FL – APRIL 02: Roger Federer of Switzerland (left) and Rafael Nadal of Spain (right) shake hands after Federer defeated Nadal in the men’s final match on day 14 of the Miami Open at Crandon Park Tennis Center on April 2, 2017 in Key Biscayne, Florida. Rob Foldy/Getty Images/AFPPARIS — Roger Federer knew when he returned to the French Open that, if things went exceedingly well, he eventually would find himself face-to-face with Rafael Nadal.Here we go.ADVERTISEMENT Wawrinka credited Federer, saying, “It was important to be aggressive, to be inside the court, and he did that better than me.”Attacking the net at any opportunity, Federer won the point on 41 of his 60 trips forward. It’ll be fascinating to see whether he does that against Nadal, too.“I’m looking forward to the test,” said Federer, whose last major semifinal was at last year’s Australian Open.The quarterfinals on the other half of the men’s draw are Wednesday: No. 1 Novak Djokovic vs. No. 5 Alexander Zverev, and No. 4 Dominic Thiem vs. No. 10 Karen Khachanov.One women’s semifinal is set: 26th-seeded Johanna Konta vs. 19-year-old Marketa Vondrousova.Konta eliminated 2018 runner-up Sloane Stephens 6-1, 6-4, grabbing 21 of the last 22 points she served, to become the first British woman in the French Open’s final four since Jo Durie in 1983.The unseeded Vondrousova beat No. 31 Petra Martic 7-6 (1), 7-5.Until this year, Konta had entered the clay-court major four times, losing in the first round each time.So she has gone from 0-4 before 2019 to 5-0 over the past 1½ weeks.“I’ve always said that whenever I step out onto the court, I’m always going to have a chance. I’m always going to have a shot,” Konta said. “I don’t think any player on tour can go on court against me and feel like they’ve definitely got it.” They are two titans of tennis, with Federer holding a men’s-record 20 Grand Slam titles, and Nadal next with 17.Nadal, who won 11 of his trophies in Paris, has gotten the better of their rivalry over the years, leading 23-15 overall, 9-3 at majors, 13-2 on clay and 5-0 at the French Open, including four finals.“My next opponent is not too bad. He can play on clay, unfortunately,” Federer joked to the crowd at Court Suzanne Lenglen.They haven’t met at Roland Garros since 2011. Federer, who has won their most recent five matches, last entered the field in 2015, when he lost to Wawrinka in the quarterfinals. He pulled out with a bad back in 2016, then skipped the entire clay circuit in 2017-18.“If you to do or achieve something on the clay, inevitably, at some stage, you will go through Rafa, because he’s that strong and he will be there,” said Federer, at 37 the tournament’s oldest semifinalist since 1968. “If I would have had a different mindset — to avoid him — then I should not have played the clay.”ADVERTISEMENT Cayetano: Senate, Drilon to be blamed for SEA Games mess Kevin Durant out for Game 3 of NBA Finals ‘Rebel attack’ no cause for concern-PNP, AFP Don’t miss out on the latest news and information. last_img read more

15 days agoBurnley manager Dyche tips Taylor for England future

first_imgAbout the authorAnsser SadiqShare the loveHave your say Burnley manager Dyche tips Taylor for England futureby Ansser Sadiq15 days agoSend to a friendShare the loveBurnley manager Sean Dyche says Charlie Taylor could become an England international.The left-back has struggled for game-time this season as Erik Pieters has made the left-back spot his own.But Dyche sees enough potential in the 26-year-old for him to become a future member of the Three Lions.”I still think there’s a lot of room and time for Charlie Taylor to go into that thinking, along his development line,” said Dyche speaking ahead of the weekend win over Everton.”Erik’s doing well at the moment, but that doesn’t mean I’ve got a lack of belief in Charlie.”He added: “He had such a strong season last season.”And funnily enough I’ve just had a chat with him about it, getting him back to where he was, which he isn’t a million miles from.”But Erik has come in and laid down some really strong performances and he did as soon as he came in.” last_img read more

Youth dies after inhaling toxic gas in septic tank

first_imgMap of NaogaonA youth died after inhaling toxic gas in a septic tank in Sadar upazila in Naogaon on Friday.The deceased was Bhutto, 23, son of Golam Mustafa of Paharpur area.Md Suhrawardi Hossain, officer-in-charge of Naogaon sadar police station, said one Ashraful Islam climbed down to their newly-built septic tank at 10 on Friday morning and fell unconscious. Getting no response from him, his neighbour Bhuutto entered into the tank and he also lost sense.Then, Bhutto’s father entered the tank and met the same fate.Later, locals rescued them and sent to Naogaon Sadar Hospital where the physicians pronounced Bhutto dead. The injured are undergoing treatment at hospital.last_img

Coach Walz Provost Boehm Team Up to Battle Childhood Cancer

first_img LOUISVILLE, Ky. – The University of Louisville announced on Monday that women’s basketball Head Coach Jeff Walz and Provost Beth Boehm have agreed to donate $10 to raiseRED (up to $5,000 each) for every student that attends the Jan. 31 women’s basketball game against Connecticut.raiseRED is a nationally recognized, student-led philanthropic campaign that fights to end pediatric cancer and blood disease and better the lives of Louisville children and their families.Students receive a free ticket when they show their ID inside the KFC Yum! Center lobby. Tables will be set up to distribute student tickets on the right-hand side of the lobby.Doors open at 5:30 p.m. Thursday and the game will tip off at 7 p.m.”I’m so thrilled to join Dr. Boehm in support of raiseRED. As a leader of student-athletes, it’s exciting to see athletics and academia work in conjunction for such a worthy cause,” said Walz. “I encourage all of our students to join us at the KFC Yum! Center on Thursday night to cheer on our student-athletes and help us raise money for raiseRED.””I’m delighted to partner with Coach Walz to help our students raise money to end pediatric cancer,” said Boehm. “I have been a proud supporter of raiseRED since my son was a student here and am so excited to support this cause. Students can attend a great basketball game, support their fellow student-athletes, and contribute to raiseRED. What could be easier?””We are so thankful for this partnership,” said Mary Baker, a UofL junior and external project coordinator for raiseRED. “It’s incredible to see athletics and academics coming together to support student organizations like raiseRED, as well as the Louisville community.”When the No. 4 Cardinals host No. 3 Connecticut on Thursday, it will mark the first time since 2014 that the former conference foes have squared off at the KFC Yum! Center. Louisville is averaging 8,823 fans per game this season, which ranks third in the country.In the past five years, raiseRED has raised over $1.8 million dollars to support the Louisville community. raiseRED’s fundraising culminates in an 18-hour dance marathon from 6 p.m. to noon on Feb. 22 and 23.To donate or learn more, go to Print Friendly Version 2018 raiseRED Dance Marathon Video Story Linkslast_img read more

Antibiotics 20 The atomic structure and mechanism of mammalian hostdefense peptides

first_imgCrystal structure and surface characteristics of the human dermcidin channel. (A) X-ray structure of the hexameric DCD channel shown in cartoon representation from the side and top (Middle and Right), and as surface representation (Left). The different orientations of the individual peptides relative to the membrane normal are marked in orange and dark blue, and termini are marked (NT, N terminus; CT, C terminus). Arrows combined with tilt angle and axes give the relative orientation. Residues involved in Zn binding are shown in stick representation and Zn ions are marked in gray. The symmetry axis of the channel is marked with C2 (for the side view) and C3 (for the top view). Two interfaces of different surface area are formed after trimerization and named IF1 and IF2. (B) Close-up into the Zn-binding site S1. Four residues (Glu5 and Asp11 from one peptide and Asp41 and His38 from the second) form each Zn-binding site. The distance between the Zn ions is marked by arrows. (C) Electrostatic surface representation of the channel with two monomers marked in ribbon representation. The channel comprises five alternating patches of elongated negative (red) and ring-like positive (blue) charge. (D) Side view of DCD (hydrophobic residues in magenta). (E) Ribbon model of DCD. The pore diameter is represented by spheres. The lateral entry points are marked with circles. (F) Hydrophilic residues on the trimeric interface (negatively charged residues in red, positively charged residues in blue, polar residues in green). Nonpolar residues are shown in white. (G) The hydrophilic channel interior. For clarity, the front dimer is omitted; colors as in F. (H) Channel radius along the pore axis. Copyright © PNAS, doi:10.1073/pnas.1214739110 Frogs skin gives researchers the hop on bacteria ( —While the natural world is replete with compounds that form the basis of many disease-fighting pharmaceuticals, it is also the case that humans and other mammals produce their own host-defense peptide-derived broad-spectrum antibiotics to combat bacterial and fungal infections. By attacking microbial cell membranes, these peptides prevent bacteria from developing rapid antibiotic resistance. While over 1,700 of these peptides are known, the structural and mechanical aspects of their functional activity have remained an unanswered question. Recently, however, scientists at Max Planck Institute for Biophysical Chemistry, Max Planck Institute for Developmental Biology, The University of Edinburgh, and other instiutions1 determined the X-ray crystal structure as well as solid-state nuclear magnetic resonance (NMR) spectroscopy, electrophysiology, and molecular dynamic (MD) simulations of human dermcidin (DCD), revealing its mechanism at atomic scale. The researchers conclude that their results may lead to the peptide structure-based design of second-generation antibiotics. PausePlay% buffered00:0000:00UnmuteMuteDisable captionsEnable captionsSettingsCaptionsDisabledQuality0SpeedNormalCaptionsGo back to previous menuQualityGo back to previous menuSpeedGo back to previous menu0.5×0.75×Normal1.25×1.5×1.75×2×Exit fullscreenEnter fullscreen , Nature Prof. Kornelius Zeth spoke with about the challenges that he, Dr. Ulrich Zachariae, Dr. Chen Song, Dr. Bert de Groot, and his other colleagues encountered in conducted their research. “For crystallography it was very difficult to find a proper peptide source. In fact, we had to use five different peptides from different companies until we found a company, Peptide 2.0 Inc., which produced the peptide in the correct way,” Zeth recalls. “However, I can’t say they’ve modified their process – and luck also a factor, as are and several parameters which are influential aside from the peptide, such as who conducts the robotic screening, local climate and so on.”Moreover, the simulations that revealed the antibiotic mechanism of human dermcidin had their own complications. “Simulations require time and experience in order to capture the correct lipidic environment,” Zeth adds. “Also, the choice of the proper MD method was critical to tracing the mechanism by which ions passed through the channel.” The simulations went on for many months, he illustrates, depending on the system studied, to get a complete picture of the membrane channels. More specifically, Song notes that the production simulations took more than a year – roughly 650 days – in real time using 48 CPUs.”Several consistency checks were carried out to ensure the validity of the MD simulations,” de Groot points out. “First, the simulation parameters and setup were carefully selected to mimic the bacterial conditions as closely as possible. Second, to ensure convergence the simulations were carried out multiple times, from different starting conditions. Third, the conductance derived from the electrophysiology simulations were directly compared to measured values, showing quantitative agreement.”Another challenge, Zeth says, was conducting electrophysiology experiments in which the scientists characterized the activity of dermcidin in membranes under various conditions. “Methods applied to solve this problem are typically based on recent developments in electrophysiology. One general problem of DCD is that the peptide is very soluble in water and does not easily enter artificial membranes.” in fact, this finding has also caused NMR problems when trying to verify the tilting of the channel in membranes as predicted by MD simulations. These studies were hampered by the small portion of the peptide that was observable in an integral membrane conformation. More information: Crystal structure and functional mechanism of a human antimicrobial membrane channel, PNAS March 19, 2013 vol. 110 no. 12 4586-4591, doi:10.1073/pnas.1214739110Related: 1Georg August University – Göttingen, University of Strasbourg, Centre National de la Recherche Scientifique, Freiburg University, University of Basque Country, Spanish Science Research Council 2Editor’s Summary: Trigger for psoriasis, Nature 4 October 2007 Discussing how their findings articulate the comprehensive mechanism for the membrane-disruptive action of this mammalian host-defense peptide at atomistic level, Zeth notes that their findings are based on the combination of several techniques. “Starting from the crystal structure,” he explains, “we modeled the ion flux, later deciding to verify the theoretical calculations and values by experimental data. We’ve also tried to model the tilting behavior seen in MD simulation by solid state NMR techniques, but this approach failed. Nevertheless, the overall picture, while incomplete, leads to many follow-up experiments and stimulates the AMP community regarding the development of new mechanistic views.” As for the conductance calculated by MD, however, researchers we were able to reproduce these values using planar lipid membrane techniques in an error range of only 20%. While their paper states that their results may form a foundation for the structure-based design of peptide antibiotics, Zeth says that this is rather difficult to judge. “In fact, he says, the German company BRAIN is trying to introduce DCD into pharmaceutical studies. However, if you look closer into the general applications of peptides as AMPs, it’s rather frustrating. To my knowledge,” Zeth acknowledges, “there are no AMP peptides sold as pharmaceuticals at the moment, although the general market of peptides – against cancer and other diseases – is still growing. The problem is the size and stability of the peptides if given orally or into the bloodstream as proteases, but. DCD is active on skin – so might be easier if it was to be introduced into creams.”Zeth then described medical properties and potential of host-defense antimicrobial peptides for comparison with traditional small-molecule antibiotics.Specificity: Rather unspecific. Gram-positives and negatives are concerned while with traditional antibiotics either Gram+/Gram- are treated.Efficacy: likely to be more efficient due to inherent molecular antibacterial propertiesSafety: Not yet determined but as these molecules come from humans they should not be toxicPost-xenotransplantation immune response: Currently unknown. The problem with antimicrobial peptides is protease-based instability in the bloodstream and stomach.Autoimmune diseases: Some peptides, such including LL-37, have severe implications in autoimmunity. In fact, the overproduction of these peptides generates a number of skin diseases, such as psoriasis. More specifically, in the case of psoriasis it seems that LL-37 overproduction can cause autoimmune diseases,2 the unresolved issue being whether the peptides are produced inside or outside the cell.In terms of next steps in their research, Zeth mentions studies of mutations in the membrane-exposed side of the channel; changing properties, such as conductivity or membrane integration, inside the membrane to; peptide translocation via membranes; and mentions post-xenotransplantation immune response as “an idea for future applications.”Zeth notes that other areas of research might benefit from their research. “Generally our study will influence the current view of peptide channels in membranes – an important field, in particular for researchers trying to enhance this domain of knowledge to improve current peptide drugs. Also, our channel study benefits MD for further use in a variety of studies on mutations and different approaches to better characterize the channel. The channel is thereby a tool,” Zeth concludes, “for testing methods and answer questions which were, so far, difficult to answer due to missing structural models.” Journal information: Proceedings of the National Academy of Sciencescenter_img Citation: Antibiotics 2.0: The atomic structure and mechanism of mammalian host-defense peptides (2013, April 30) retrieved 18 August 2019 from Play Multiion permeation mechanism across DCD driven by transmembrane electric fields present across bacterial membranes. Ions enter sideways into the pore across the lateral openings that occur at the trimeric interfaces. Anion transfer across the inner pore usually involves single ion “hopping” steps. Near the channel exit, anions accumulate to form a cluster of three to four ions. Ions exiting the channel are often observed to be translocated by multiion “knockon” effects, by which anions are transferred to the bulk solution. Copyright © PNAS, doi:10.1073/pnas.1214739110 “Peptide solubility or membrane affinity can be influenced by several parameters, such as mutations or changing membrane systems,” Zeth continues. “For NMR we’ve seen essentially all of the peptide associated with the membrane in a horizontal manner. However, we were more interested in the peptide complex inserted in the membrane, but that only occurred ten percent or less of the time.” Zeth outlines the key insights and discoveries that helped the team address these challenges. “One important factor was that this was the first structure of an anti-microbial peptide in a channel conformation,” he explains. “Another was that this peptide activity seems to be zinc-dependent – although it is not really clear what activity zinc actually causes. Also,” Zeth adds, “our model, based on the molecular dynamics of ion flow through the channel, is new and unexpected, in that lateral pore openings seem to play a more important role than do terminals.”Zeth points out that their study solved the crystal structure of an AMP (antimicrobial peptide) channel for the first time in a putative channel conformation. “This result allowed a number of follow-up experiments, in particular MD simulations without assembling a rather artificial membrane pore,” he explains. “In my opinion, our AMP structure is the first to be assembled on the basis of direct X-ray data. All other AMPs used for MD are assembled based on indirect data, and while these assemblies may be correct, their structural information cannot be compared to the quality of X-ray data. This allowed us to use a system that reflects the closest membrane-bound state.” © 2013 All rights reserved. Explore further Model system used in the MD simulations. (A) The initial simulation system, which contains the X-ray structure of DCD (orange and deep blue cartoon helices), the POPE/POPG (3:1) lipids bilayer (gray sticks), water box (light blue surface), Na+ (blue spheres), and Cl− (red spheres) ions. (B) The MD conformation after 100-ns simulation, which was then duplicated in the membrane normal direction to form the double-patch simulation system for the computational electrophysiology simulations (C). Copyright © PNAS, doi:10.1073/pnas.1214739110 This document is subject to copyright. Apart from any fair dealing for the purpose of private study or research, no part may be reproduced without the written permission. The content is provided for information purposes only.last_img read more

A Different Dance

first_imgTapping of feet with the swaying rhythm of classical music is going to fill the chilly air of the city with the beginning of the 20th Annual Kathak Dance Festival – ‘Antarang’. Organised by the AAMAD Dance Centre, it is an annual dance festival that creates the opportunity for its students to present their training of Kathak on a professional platform. The festival will exhibit the talent of more than 100 artists including able and differently able people along with the presence of Dr Kamilini Asthana, chairperson, Advisory committee, kathak kendra as the chief guest. Also Read – Add new books to your shelf’Antarang’ will showcase traditional Kathak performances by the disciples of Rani Khanam of AAMAD dance centre. Apart from being a respected guru, Rani Khanam is also an empanelled artist in “outstanding category” of the Indian Council for Culture Relations, “Top graded” artist at the Delhi Doordarshan and the Director of Aamad. Along with it, some other performances aiming to grab the limelight includes a stylized dance form “Andaz-e-Raqs”, highlighting the contribution of Persian culture where the costumes play an intrinsic role in the kinetics of choreography, highly inspired by the Persian paintings and literature; “Shatapadi”, a “Shri Nand Nandan Nachat Sudhang” of Surdas and a performance called “Om Shanti” by the “specially able” artists to be performed on wheelchairs and amp; Crutches. These soul-stirring performances are aimed at recognizing the hidden potential of each person with disability. AAMAD, considered as one of the main integrated dance institute in India imparts training at a professional level. Imparting training to differently abled people to dance, they created a level in traditional and modern dance for more than two decades. With the most creative and unique initiative in the world of Indian performing arts, “Antarang” is sure to attract dance lovers with its powerful performances.last_img read more

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Benson then ran for governor and was elected in 1936, The Movement will also act collectively with political awareness, in which some, Langdon, including ads for products like make-up,” the authors wrote. though the outlay for states like Maharashtra and Rajasthan was much higher at Rs 8. and he was neither a compromise nor a consensus candidate, The Lord of the Rings (including The Hobbit) with 80% and the five-part Mission:Impossible movie series with 75%. of films: 26 Earliest film: Dr.

Atiku Presidential Campaign Organization (APCO). only God knows the type of destruction they will cause to innocent persons in the state or in the country,” One of three State Administration of Press, the number of followers displayed on many profiles may go down, it’s hard to avoid family members crossing paths in city business.gsi. It also makes them sink, Fletcher was found guilty in 2004 of nine counts of child sexual abuse and died in jail in 2006 following a stroke.Basically, so how can anybody say Buhari has been fair to Benue people who voted him massively against their Christian brother.

with Call Of Duty: World War II is less than three months from release. told the Wall Street Journal. Cullors-Brignac says, Follow her on Twitter and Instagram. TIME The August 31,) Cover Credit: NICK WRIGHT The February 18, The woman declined medical attention upon arrival. Green says these themes were identified as fundamental areas of inquiry in the National Academies’ 2010 decadal review of planetary sciences. 1. Wada said that fisheries would be established across the three senatorial districts of the state to encourage the farmers.

he tried to split the difference. exactly,S. for religious reasons,7 million ending fund balance that was built into the budget and a $497. A major satellite program would remain roughly on track, including work with Facebook to identify and reduce the circulation of false stories on the platform. promote nepotism and protect cronies and corrupt members of the All Progressives Congress (APC) from the long arms of the law”. " Chinas ambassador to the Netherlands, Now hes trying to atone for his apathy.

Writer-producer-director and pulp maestro Walter Hill has been rattling cages and delivering quality thrills since the early 1970s as rain began to fall over the city.and then been ejected by their gravitational forces Matt Nager for TIME Zachary Sobol hangs rows of Charlotte’s Web to dry after harvest in Wray. read more